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KMID : 0613820120220040508
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Journal of Life Science
2012 Volume.22 No. 4 p.508 ~ p.515
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Immobilization of Yeast Pichia stipitis for Ethanol Production
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Lee Sang-Eun
Lee Ji-Eun
Kim Eun-Jin
Choi Joon-Ho
Choi Woon-Yong
Kang Do-Hyung
Lee Hyeon-Yong
Jung Kyung-Hwan
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Abstract
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In this study, DEAE-cotton [derivatized by 2-(diethylamino)ethyl chloride hydrochloride (DEAE HCl)] was prepared as a carrier for immobilized Pichia stipitis for ethanol production. When cotton was derivatized with 0.5 M DEAE HCl, the yeast cell suspension was adsorbed at 100% of the initial cell OD_600. The adsorbed yeast cells were estimated to be 101.8 mg-dry cells/g-DEAE-cotton. In particular, when a flask culture using the immobilized yeast cells was conducted in a glucose and xylose-containing medium, the yeast cells on the DEAE-cotton gradually produced ethanol, according to glucose and xylose consumption; the ethanol yield was approximately 0.33 g-ethanol/g-monosaccharide. Because DEAE-cotton was successfully used as a carrier for ethanol production from a glucose and xylose-containing medium, we expect that this bioethanol production process may be used for the bioethanol production process from the hydrolysate of lignocellulosic biomass. All the results of DEAE-cotton were compared with those of DEAE-cellulose as a carrier for immobilization.
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KEYWORD
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Cotton, DEAE.HCl derivatization, yeast cell immobilization, ethanol production, Pichia_stipitis
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